NEXTERION® Streptavidin coating (HS)

The NEXTERION® Streptavidin coating is specifically developed for binding all types of biotinylated molecules. Biotin-tagged molecules are immobilized in a directional way to the streptavidin molecules and applied onto the glass substrate.

Product information

The proprietary thin-film deposition coating process produces a very high density, as well as an extremely uniform coating of streptavidin molecules. The resulting surface has a very high binding capacity combined with a low signal variance across a single slide as well as different slides and batches. Produced in ISO class 5 clean room conditions using a standardized process and running a stringent quality control system, streptavidin coated substrates are available in standard and custom formats

Shelf life

Six months for sealed packages when stored at – 20° C. To protect the activity of the streptavidin layer, a protective coating is added on top of the reactive coating prior to packaging. This layer must be removed prior to use by a washing step. The streptavidin layer on Slide HS is very sensitive to elevated temperatures. It is therefore necessary to ship the product in a frozen state with dry ice. The product must immediately be put into a freezer upon arrival. Repeated freeze and thaw cycles should be avoided.

Probe types

• All molecules that can be biotinylated

Immobilization method

Binding via biotin