NEXTERION® 3-D Hydrogel coating (H)

The NEXTERION® 3-D Hydrogel coating provides the ideal surface for the covalent immobilization of biomolecules containing primary amines (NH₂ groups) such as proteins, antibodies and most peptides. Additionally, for amino-modified biomolecules like glycans or oligonucleotides this surface may be the coating of choice.

Product information

This type of coating has proven to be a very attractive alternative to the commonly used nitrocellulose-coated slides, especially when low background noise or slide transparency are important. It ensures an extremely low non-specific binding and helps preserving the native three-dimensional structure of complex bio-molecules, thus maintaining conformation and functionality. As a result, it attains excellent signal-to-background ratios. Produced in ISO class 5 clean room conditions using a proprietary thin-film deposition process and running a stringent quality control system, the 3-D hydrogel coating is offered in standard and custom formats.

Probe types

• Amino-linked glucans
• Antibodies and antibody fragments
• Functional proteins such as enzymes or receptors
• Small protein fragments such as peptides
• Amino-modified oligonucleotides 16 to 70 mers

Shelf life

Twelve months sealed packages when stored at - 20° C.

Immobilization method

Covalent binding via amine reactive chemistry.